Degree in Medical
Biotechnology - University of Bologna
ADVANCED
HUMAN GENETICS (I.C.)
Module
of CLASSICAL AND NEXT-GENERATION GENOMICS
- A.Y. 2024/2025
Teacher: Pierluigi Strippoli
Teaching Material 2024/2025 in Virtuale
Paper vs. Pixels
1.
Human
Genomics
[Source for the exam: Chapters #1, #2 and #3 only
from Information - Gene
- Genome Lecture Notes
in Virtuale]
2. Bibliographic
Databases
3. Nucleic Acid Extraction
[Source
for the exam: Nucleic
Acids Extraction principles]
4. Nucleic
Acid
Hybridization -
Concept of Probe
Specificity - Southern
Blot - Northern
Blot -
Microarray
[Source
for the exam:
Nucleic
Acid
Hybridization principles;
and the
section
"Principle of
method" in
this review: Expression
Profiling]
5.
Polymerase
Chain Reaction
(PCR)
- Primer design [Example
Article] -
Reaction
Kinetics
[Source
for the exam: PCR
principles]
6. Genetic
Libraries - RNA
Vaccine
7. Nucleic
Acid Sequencing
and Sequence
Analysis
8. System
Biology -
The case of trisomy
21: a model for
integrated research
FILMOGRAPHY
Ron Howard
(Director). Apollo
13.
With Tom Hanks,
Ed Harris. USA,
1995.
["Houston, we
have a problem".
A true story.
The positive
hypothesis when
dealing with a
problem].
George
Miller
(Director). Lorenzo's
Oil.
With Nick Nolte,
Susan Sarandon.
USA, 1992.
[The true story
of two parents
of a child with
adrenoleukodystrophy -
X-linked disease
- who discover a
diet therapy].
Andrew Niccol
(Director). Gattaca.
With Ethan
Hawke, Uma
Thurman. USA,
1997.
["There is no
gene for the
human spirit"].
***
Laboratory
Lab 1 - Data
Banks
Lab 2 - Gene
Structure - Lab Exercise
Lab 3 - PCR Primer - Example
Article (MTHFR) - Example Article [COVID-19] - In-Silico PCR
Lab 4 - Data
Analysis
Lab 5 - Documentary
DNA